Research Protocols

Protocols for species dissection:

• The spotted lanternfly, Lycorma delicatula
• The spotted wing drosophila, Drosophila suzukii
• Grasshoppers of the Acrididae family

Insect collection and feeding experiments for molecular gut content analysis:

• Feeding experiments with grasshoppers
• Collection of the spotted lanternfly and host plant samples

DNA barcoding protocols:

• Overview of the main DNA barcoding steps
• DNA extraction
• Primer dilutions
• PCR protocols: (a) isopods; (b) leafhoppers; (c) spotted lanternfly
• PCR product purification
• Gel electrophoresis
• Sanger sequencing: sequence editing and alignment
• Next-generation sequencing: (a) sample preparation and submission; (b) retrieving sequences from GENEWIZ